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Cigarette smoke extracts (CSE) stocks preparation and grouping: Given the different contents of individual constituents among the experimental cigarettes and 3R4F cigarettes, nicotine was added to the CSE of denicotinized cigarettes (named DNC) to a final concentration that was consistent with the nicotine content of 3R4F cigarette CSE (named DNC + NIC) and represented the normal nicotine content of cigarettes